THIOBACILLUS DENITRIFICANS MEDIUM I

Medium: 
162

THIOBACILLUS DENITRIFICANS MEDIUM I

Solution A:

KH2PO4                                                                               2.0 g

KNO3                                                                                   2.0 g

NH4Cl                                                                                  1.0 g

MgSO4 x 7 H2O                                                                   0.8 g

Trace element solution SL-4 (see below)                            2.0 ml

Distilled water                                                                  960.0 ml

Adjust pH to 7.0 with NaOH.

 

Solution B:

Na2S2O3 x 5 H2O                                                              5.0 g

Distilled water                                                                   20.0 ml

 

Solution C:

NaHCO3                                                                               1.0 g

Distilled water                                                                    20.0 ml

 

Solution D:

FeSO4 x 7 H2O                                                                    2.0 mg

H2SO4 0.1 N                                                                        1.0 ml

Solutions A, B and D are sterilized separately by autoclaving at 121°C for 15 min.

Solution C is sterilized by filtration.

 

solutions B to D are added to the sterile solution A in the sequence as indicated.

Note: A small amount of white precipitate forms after autoclaving, but this has no negative effect on growth.

 

Trace element solution SL-4: 

Na2-EDTA                                                           0.50 g

FeSO4 x 7 H2O                                                  0.20 g

ZnSO4 x 7 H2O                                                  0.10 g

MnCl2 x 4 H2O                                                   0.03 g

H3BO3                                                                0.30 g

CoCl2 x 6 H2O                                                    0.20 g

CuCl2 x 2 H2O                                                    0.01 g

NiCl2 x 6 H2O                                                     0.02 g

Na2MoO4 x 2 H2O                                             0.03 g

Distilled water                                               1000.00 ml

 

First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.