Department:
Pharmaceutical & Organic Chemical Technology
Project manager(s):
Year:
2016
| soya (max Glycine) is one of the enrich source of phytoesterogenes. Isoflavones have a similar sturucture and function like estrogen in reducing menopausal symptoms and their positive impact have been proved. In the present study extraction of isoflavones from soybeans with ethanol 96% by four different techniques including soxhlet, multi-stage solvent extraction, pre-treated sample by ultrasound and microwave prior to multi-stage solvent extraction carried outtt and efficient parameters in each methods were optimized. The results showed Soxhlet extraction of 50 g the sample with 150 ml ethanol 96%, during 6h the maximum extraction yield of phenolics and isoflavanoids were obtained 68.62% and 62.02% respectively. Multi-stage solven extraction performed by experimenttal conditions the ratio of solids to solvent extraction 3/10 g.ml-1, 45C temperature and extraction time 60 min (per extraction), so in this conditions the extraction yield were same as Soxhel mehtod. In pretreated samples by ultrasound prior to three-stage solvent extraction (for 40 min in cycle 1 and amplitude 60) and microwave-pretreated samples (time leaching of 20 min and radiation time of 50 min), the maximum extraction yield compare to soxhlet method was obtained in solid to solvent ratio of 3.10 g.ml-1, temperature of 40C, extraction time of 15 min. Also qualitative and quantitative analysis of flavonoids genistein and daidzein in alcoholic dry extract was determined by HPLC. |