Cancer is a leading cause of death worldwide. Many heterocyclic cores are present in the structures of clinically approved anticancer drugs. Meanwhile, benzothiazoles have been reported as one of the most important heterocyclic scaffolds in previously reported anticancer agents in the literature. Therefore, in this report, a novel series of 2-phenyl benzothiazole derivatives was synthesized, biologically evaluated against breast cancer cell line (T47D) and compared with etoposide as a reference drug.  The anticancer activities were evaluated by MTT colorimetric assay. Among all tested compounds, N-(4-(6-methoxybenzo[d]thiazol-2-yl)phenyl)acetamide illustrated the most potent cytotoxic activity.